Amplex Red Dye Profile

561

Laser Line

585/42

Common Filter

569

Excitation Max

581

Emission Max

About Amplex Red


10-Acetyl-37-dihydroxyphenoxazin (Amplex Red) is a fluorogenic substrate that is stoichiometrically converted in the presense horseradish peroxidase (HRP) and H2O2. This reagent is commonly used in Microplate Assay or to detect an HRP cojugated antibody in western blot. It has an excitation peak at 569 nm and an emission peak at 581 nm.



Spectra