CellBrite Fix 640 Dye Profile

Biotium™

628-640

Laser Line

660/20

Common Filter

638

Excitation Max

667

Emission Max

About CellBrite Fix 640


Biotium's CellBrite Fix dyes are versatile amine-reactive dyes that are primarily used for live cell outer plasma membrane staining but can withstand fixation and detergent permeabilization. The unique benefit here is that since CellBrite Fix dyes can withstand permeabilization, extracellular staining can be combined with intracellular staining techniques. This dye line shares similarities with Biotium's MemBrite Fix and MemBrite Fix-ST line, but there are a few key differences. CellBrite Fix stains are easy to use and require a single staining step while MemBrite Fix dyes require a two-step staining protocol. While ease of use is important, MemBrite Fix and Membrite Fix-ST dyes have more colors to choose from and they do not associate with lipids in the membrane which leads to lower cytoplasmic background after permeabilization.



Spectra



Dyes similar to CellBrite Fix 640 based on emission

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APC ATTO 647 ATTO 647N Alexa Fluor 647 Brilliant Blue 660-P2 BDY 650-X SE BODIPY 650/665 Brilliant Ultraviolet 661 BactoView Live Red CF640R CF647 CF647ST CF650 CPN680 Red Calcein Deep Red AM CellBrite Red CellBrite Steady 650 CellROX Deep Red CellTrace Far Red CellVue Maroon CellVue Plum Chromeo 642 CoraLite Plus 647 Cy5 Cyanine 5 SE Cyanine 647 CypHer5E DY-481XL DY-520XL DY-521XL DiD DyLight 633 DyLight 649 DyLight 650 Dyomics 647 ER-ID Red ExoBrite 640/660 EV Membrane Stain FLUX 640 FLUX 647 FVS660 Flash Phalloidin NIR 647 FluoProbes647H Fura Red AM (Ca2+bound) Fura Red AM (Ca2+free) FxCycle Far Red HiLyte 647 IRDye 650 Janelia Fluor 646 LipidSpot 610 Live-or-Dye 640/662 Live/Dead Fix Far Red LysoView 633 LysoView 640 MaxLight 650 MemBrite Fix 640/660 MemBrite Fix ST 650/665 MitoView 650 NL 637 NeuroVue Maroon Nile Blue NirFP NovaFluor Blue 660 / 120S NovaFluor Blue 660 / 40S NovaFluor Red 660 NovaFluor Yellow 660 NucSpot 640 NucSpot Far-Red Oyster 650 SureLight P3 PE-Alexa 647 PE-Cy5 PE-Dyomics 647 PerCP PhenoVue Fluor 647 PhenoVue Fluor 647 Live Cell PhenoVue ROS-670, Total Oxidative Stress Indicator PhenoVue 641 Mitochondrial Stain PromoFluor 647P ROS-ID Red RPE-CF647T ReadiLink 647-674 STAR 635 STAR RED Seta-650-NHS Seta-PerCP-680 Seta-R-PE-670 StarBright Blue 675 StarBright UltraViolet 665 StarBright Violet 670 StarBright Yellow 665 SureLight APC SureLight PE Cyanine 5.0 TO-Pro 3 TOTO-3 TSA Vivid 650 Thiazole Red Homodimer Tri-Color Vio667 VioBright 667 VivaFix 649/660 eFluor 660 eFluor 670 Cell Proliferation iFluor 647 iRFP 670