NucSpot Far-Red Dye Profile

Biotium™

561-594

Laser Line

660/20

Common Filter

596

Excitation Max

667

Emission Max

About NucSpot Far-Red


Biotium's NucSpot Far-Red is a cell membrane-impermeant dye that enters cells with compromised membranes and is used for live/dead discrimination or DNA content analysis. This dye was developed as an alternative to 7-AAD with more advantageous spectral properties for reduced spillover. Since this dye stains cells with compromised membranes, it can be used with fixed cells, permeabilized cells, or as a stain for dead cell detection with flow cytometry and fluorescence microscopy applications. It has an excitation peak at 596 nm and an emission peak at 667 nm.



Spectra



Dyes similar to NucSpot Far-Red based on emission

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APC ATTO 647 ATTO 647N Alexa Fluor 647 Brilliant Blue 660-P2 BDY 650-X SE BODIPY 650/665 Brilliant Ultraviolet 661 BactoView Live Red CF640R CF647 CF647ST CF650 CPN680 Red Calcein Deep Red AM CellBrite Fix 640 CellBrite Red CellBrite Steady 650 CellROX Deep Red CellTrace Far Red CellVue Maroon CellVue Plum Chromeo 642 CoraLite Plus 647 Cy5 Cyanine 5 SE Cyanine 647 CypHer5E DY-481XL DY-520XL DY-521XL DiD DyLight 633 DyLight 649 DyLight 650 Dyomics 647 ER-ID Red ExoBrite 640/660 EV Membrane Stain FLUX 640 FLUX 647 FVS660 Flash Phalloidin NIR 647 FluoProbes647H Fura Red AM (Ca2+bound) Fura Red AM (Ca2+free) FxCycle Far Red HiLyte 647 IRDye 650 Janelia Fluor 646 LipidSpot 610 Live-or-Dye 640/662 Live/Dead Fix Far Red LysoView 633 LysoView 640 MaxLight 650 MemBrite Fix 640/660 MemBrite Fix ST 650/665 MitoView 650 NL 637 NeuroVue Maroon Nile Blue NirFP NovaFluor Blue 660 / 120S NovaFluor Blue 660 / 40S NovaFluor Red 660 NovaFluor Yellow 660 NucSpot 640 Oyster 650 SureLight P3 PE-Alexa 647 PE-Cy5 PE-Dyomics 647 PerCP PhenoVue Fluor 647 PhenoVue Fluor 647 Live Cell PhenoVue ROS-670, Total Oxidative Stress Indicator PhenoVue 641 Mitochondrial Stain PromoFluor 647P ROS-ID Red RPE-CF647T ReadiLink 647-674 STAR 635 STAR RED Seta-650-NHS Seta-PerCP-680 Seta-R-PE-670 StarBright Blue 675 StarBright UltraViolet 665 StarBright Violet 670 StarBright Yellow 665 SureLight APC SureLight PE Cyanine 5.0 TO-Pro 3 TOTO-3 TSA Vivid 650 Thiazole Red Homodimer Tri-Color Vio667 VioBright 667 VivaFix 649/660 eFluor 660 eFluor 670 Cell Proliferation iFluor 647 iRFP 670