About Alexa Fluor 555

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Alexa Fluor™ 555 (AF555, Alexa 555) has an excitation peak at 555 nm and an emission peak at 565 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC, iFluor® 555 (ATT Bioquest) and iFluor® 560 (ATT Bioquest). Many flow cytometers are equipped with 532nm lasers, so while Alexa 555 can be used for flow cytometry, PE is more commonly used due to its brightness and spectral characteristics. This dye is commonly used for fluorescence microscopy applications.

Alternative Dyes to Alexa Fluor 555

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Cy3 ATTO 550 CoraLite Plus 555

Dyes similar to Alexa Fluor 555 based on emission

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ATTO 542 ATTO Rho6G Alexa Fluor 546 BDY TMR-X SE BODIPY 558/568 BODIPY TMR-X BPE Brilliant Ultraviolet 563 Brilliant Violet 570 CF532 CF535ST CF540 CF543 CF555 Calcein Red Calcein Red AM Calcium Orange CellBrite Fix 555 CellBrite Orange CellMask Orange Plasma Membrane Stain CellROX Orange CellTracker Orange Chromeo 546 CoraLite Plus 555 Cy3 Cyanine 3 SE Cyanine 555 CytoTrack Yellow DY-395XL DY-485XL DiI Dyomics 547 EFLUXX-ID Gold FVS575V HiLyte 555 Janelia Fluor 549 LIVE 550 LSSmOrange Live/Dead Fix Olive LysoView 550 MITO-ID Green MemBrite Fix 543/560 MemBrite Fix 543/560 MitoLite Orange FX570 MitoTracker Orange NL 557 NeuroVue Orange NovaFluor Yellow 570 NucView 530 OC515 OFP Oyster 550 PKH26 POPO-3 PhenoVue 512 Nucleic Acid Stain – complexed with nucleic acids PhenoVue ROS-570, Total Oxidative Stress Indicator PhenoVue 551 Mitochondrial Stain PhenoVue Fluor 555 PromoFluor 555P Pyronin Y Qdot 565 ReadiLink 555-570 Rhod-2 (Ca2+ bound) Rhodamine B Seta-555-NHS Spark Blue 574 Spark YG 570 StarBright UltraViolet 575 StarBright Violet 570 SureLight BPE Sytox Orange TFAX 546 SE TMRE ViaKrome 561 VivaFix 547/573 Zombie Yellow eFluor 565NC eFluor 570 iFluor 532 iFluor 555 mKO mKusabira-Orange2 mOrange BOP-JF549