About SNARF 1 pH6.0

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SNARF-1, AM is a cell-permeant pH probe with a pKa of 7.5 after hydrolysis of the acetoxymethyl ester of the probe upon entering live cells. SNARF-1 is a ratiometric indicator sensitive to measuring pH changes between pH 7 and pH 8 and shifts from 580 nm under acidic pH to 640 nm under basic conditions. It also undergoes a shift in its excitation peak between pH6 to pH9 from ~532 nm at pH6 to ~561nm at pH9, but typically this probe is typically used in fluorescent imaging applications with a single excitation peak at 555nm to monitor the dual emission shift.

Dyes similar to SNARF 1 pH6.0 based on emission

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10-Acetyl-37-dihydroxyphenoxazin 5-TAMRA 6-TAMRA ATTO 550 ATTO 565 ATTO Rho3B Alizarin Red Amplex Red CF550R CF568 CF568ST CF570 CMA-Chromomycin A3 Calcein Red-Orange AM CellBrite Steady 550 CellTrace Yellow Cy3.5 CytoTrace Red DY-510XL DsRed Express DsRed2 DyLight 549 DyLight 550 EMA ExoBrite 560/585 EV Membrane Stain FVS570 GelRed Kaede (red) KikGR1 (red) LIVE 560 LipidSpot 488 Live-or-Dye 568/583 Live-or-Dye NucFix Red MITO-ID MP (high potential) MagDot 575 MaxLight 550 MemBrite Fix 568/580 MultiDot 575 NeuroVue Red NovaFluor Blue 585 NovaFluor Yellow 590 PE PROTEOSTAT-Red PhenoVue Cal-590 AM Bright, Calcium Indicator PhenoVue Cal-590 AM, Calcium Indicator PhenoVue Fluor 400LS PhenoVue Fluor 555 Live Cell PhenoVue 512 Nucleic Acid Stain PhenoVue 577 Lysosomal Stain Qdot 585 RFP RealYellow 586 Resazurin Resorufin Rhod-5N (Ca2+ bound) Rhodamine Rhodamine Red-X Seta-400-NHS Spark YG 581 Spark YG 593 StarBright Blue 520 StarBright Blue 580 StarBright Yellow 575 SureLight RPE TRITC TSA Vivid 570 TagRFP VRDye 549 alamarBlue dTomato dsRed eFluor 585NC mApple mScarlet mTangerine pHrodo Red tdTomato