About Alexa Fluor 546

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Alexa Fluor™ 546 (AF546, Alexa 546) has an excitation peak at 556 nm and an emission peak at 573 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC and iFluor® 555 (ATT Bioquest). Many flow cytometers are equipped with 532nm lasers, so while Alexa 546 can be used for flow cytometry, PE is more commonly used due to its brightness and spectral characteristics. This dye is commonly used for fluorescence microscopy applications.

Alternative Dyes to Alexa Fluor 546

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TRITC ATTO 542 ATTO 532

Dyes similar to Alexa Fluor 546 based on emission

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5-TAMRA 6-TAMRA ATTO 550 Alexa Fluor 555 Amplex Red BDY TMR-X SE BODIPY 558/568 BODIPY TMR-X Brilliant Violet 570 CF535ST CF540 CF550R CF555 CMA-Chromomycin A3 Calcein Red Calcein Red AM Calcium Orange CellBrite Fix 555 CellBrite Orange CellBrite Steady 550 CellMask Orange Plasma Membrane Stain CellROX Orange CellTrace Yellow CellTracker Orange CoraLite Plus 555 Cy3 Cyanine 555 CytoTrace Red DY-395XL DiI DyLight 549 DyLight 550 Dyomics 547 FVS570 FVS575V HiLyte 555 Janelia Fluor 549 Kaede (red) LIVE 550 LSSmOrange LysoView 550 MITO-ID Green MITO-ID MP (high potential) MagDot 575 MaxLight 550 MitoLite Orange FX570 MitoTracker Orange MultiDot 575 NL 557 NeuroVue Orange NovaFluor Yellow 570 OC515 OFP Oyster 550 PE PKH26 POPO-3 PhenoVue Fluor 555 Live Cell PhenoVue ROS-570, Total Oxidative Stress Indicator PhenoVue 551 Mitochondrial Stain PhenoVue Fluor 555 PromoFluor 555P Qdot 565 ReadiLink 555-570 Resazurin Rhod-2 (Ca2+ bound) Rhod-5N (Ca2+ bound) Rhodamine Rhodamine B Seta-400-NHS Seta-555-NHS Spark Blue 574 Spark YG 570 Spark YG 581 StarBright Blue 520 StarBright Blue 580 StarBright UltraViolet 575 StarBright Violet 570 StarBright Yellow 575 SureLight BPE SureLight RPE Sytox Orange TFAX 546 SE TMRE TRITC TSA Vivid 570 TagRFP VRDye 549 ViaKrome 561 VivaFix 547/573 Zombie Yellow alamarBlue dTomato eFluor 565NC eFluor 570 iFluor 555 tdTomato BOP-JF549