Live-or-Dye 750/777 Dye Profile

Biotium™

633/750

Laser Line

780/60

Common Filter

755

Excitation Max

779

Emission Max

About Live-or-Dye 750/777


Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 750/777 has an excitation peak at 750 nm and an emission peak at 777 nm. In flow cytometry, the 633nm laser is typically used for excitation.



Spectra