About Brilliant Violet 421

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Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. Other dyes that are considered similar include SuperNova V428 (Beckman Coulter), StarBright Violet 440 (Bio-Rad) and SuperBright 436 (Thermo Fisher). BV421 is very bright and is most commonly used for flow cytometry. Its photostablity, fixation stablity, and non-toxicity properties also make BV421 useful for cell sorting and live cell Fluorescence Microscopy applications. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.

Alternative Dyes to Brilliant Violet 421

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SuperNova v428 SuperBright 436 StarBright Violet 440

Dyes similar to Brilliant Violet 421 based on emission

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Alexa Fluor 405 CellBrite Steady 405 DyLight 405 HiLyte 405 MagDot 420 MaxLight 405 MultiDot 420 PromoFluor 405 Spark Violet 423 SuperNova v428 ViaKrome 405 Vio Bright V423 Zombie Violet iFluor 405